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Registro Completo |
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
05/09/1995 |
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Data da última atualização: |
05/09/1995 |
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Autoria: |
CASTRO, V. L. S. S.; CAPALBO, D. M. F.; SOARES, C. M.; COSTA, F. P. |
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Afiliação: |
EMBRAPA-CNPMA. |
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Título: |
Identification of adverse effects of bacterial agents in mammals. |
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Ano de publicação: |
1995 |
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Fonte/Imprenta: |
In: INTERNATIONAL SYMPOSIUM ON MICROBIAL ECOLOGY, 7., 1995, Santos. Abstracts. Sao Paulo: Sociedade Brasileira de Microbiologia, 1995. |
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Páginas: |
n.p. |
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Idioma: |
Inglês |
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Conteúdo: |
A protocol of toxicological tests for registration of microbial pest-control agents (MPCA) has been recently proposed in Brazil. Its objective is to determine detrimental effects to humans and domestic animals, and should be performed in a sequence of three tiers. The first one provides an evaluation of the potential risks of the MPCA due to pathogenicity, infectivity and toxicity by an acute exposition. A standard methodology for such tests is needed, and it was the concern of this study. Rats were housed in metabolic cages at controlled conditions. The MPCA Bacillus thuringiensis var. kurstaki (Bt) was gavage or instilled in one dose containing 10.8 spores. It was conducted periodical clinical and microbial observations after dosing. When no Bt was noticed the feces anymore, gross necropsy of all animals was conducted. The rats have not presented neither clinical or pathological alterations nor body weight loss. The protocol hereby described showed to be useful for the mean purpose. It could be followed to bacterial agents as an easily make one instead of immunological assays or DNA probes to estimate clearence of the agent after the administration in the feces. |
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Palavras-Chave: |
Bioinsecticide; Crop protection; Protocol; Toxicological test. |
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Thesagro: |
Bacillus Thuringiensis. |
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Thesaurus Nal: |
environmental impact; risk analysis; risk assessment. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01986naa a2200265 a 4500
001 1012304
005 1995-09-05
008 1995 bl uuuu u00u1 u #d
100 1 $aCASTRO, V. L. S. S.
245 $aIdentification of adverse effects of bacterial agents in mammals.
260 $c1995
300 $an.p.
520 $aA protocol of toxicological tests for registration of microbial pest-control agents (MPCA) has been recently proposed in Brazil. Its objective is to determine detrimental effects to humans and domestic animals, and should be performed in a sequence of three tiers. The first one provides an evaluation of the potential risks of the MPCA due to pathogenicity, infectivity and toxicity by an acute exposition. A standard methodology for such tests is needed, and it was the concern of this study. Rats were housed in metabolic cages at controlled conditions. The MPCA Bacillus thuringiensis var. kurstaki (Bt) was gavage or instilled in one dose containing 10.8 spores. It was conducted periodical clinical and microbial observations after dosing. When no Bt was noticed the feces anymore, gross necropsy of all animals was conducted. The rats have not presented neither clinical or pathological alterations nor body weight loss. The protocol hereby described showed to be useful for the mean purpose. It could be followed to bacterial agents as an easily make one instead of immunological assays or DNA probes to estimate clearence of the agent after the administration in the feces.
650 $aenvironmental impact
650 $arisk analysis
650 $arisk assessment
650 $aBacillus Thuringiensis
653 $aBioinsecticide
653 $aCrop protection
653 $aProtocol
653 $aToxicological test
700 1 $aCAPALBO, D. M. F.
700 1 $aSOARES, C. M.
700 1 $aCOSTA, F. P.
773 $tIn: INTERNATIONAL SYMPOSIUM ON MICROBIAL ECOLOGY, 7., 1995, Santos. Abstracts. Sao Paulo: Sociedade Brasileira de Microbiologia, 1995.
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Registro original: |
Embrapa Meio Ambiente (CNPMA) |
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